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tie2 kinase inhibitor bay 826  (Tocris)


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    Structured Review

    Tocris tie2 kinase inhibitor bay 826
    Tie2 Kinase Inhibitor Bay 826, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bay+826/BAY+826/pm41891922-351-54-61
    Average 94 stars, based on 6 article reviews
    tie2 kinase inhibitor bay 826 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Angiopoietin-1 Upregulates Cancer Cell Motility in Colorectal Cancer Liver Metastases through Actin-Related Protein 2/3
    Article Snippet: .. We also treated the cells with either 0.4 μM BAY-826 (Tocris Bioscience, Toronto, ON, Canada, #6579) or 2 μM LY294002 (Selleckchem, Burlington, ON, Canada, #S1105) for 24 h to block Tie2 or PI3K/AKT pathway in the cells. .. We also treated the control cells with DMSO (Tocris Bioscience, Toronto, ON, Canada, # 3176) based on the concentration of the used inhibitors.

    Inhibition:

    Article Title: Synaptotagmin-like protein 2a regulates lumen formation via Weibel-Palade body apical secretion of angiopoietin-2 during angiogenesis
    Article Snippet: .. For Tie- 2 inhibition, cells were treated with BAY-826 (TOCRIS) at a final concentration of 1.3 nM for 1-3 days during sprouting. ..

    Concentration Assay:

    Article Title: Synaptotagmin-like protein 2a regulates lumen formation via Weibel-Palade body apical secretion of angiopoietin-2 during angiogenesis
    Article Snippet: .. For Tie- 2 inhibition, cells were treated with BAY-826 (TOCRIS) at a final concentration of 1.3 nM for 1-3 days during sprouting. ..

    Phospho-proteomics:

    Article Title: Angiopoietin–TIE2 feedforward circuit promotes PIK3CA-driven venous malformations
    Article Snippet: .. To stimulate TIE2 phosphorylation, rhANGPT1 (R&D Systems, 923-AN-025/CF) was reconstituted in PBS and used at the concentrations of 50 ng ml −1 (PIK3CAH1047R expression, Fig. ) or 200 ng ml −1 (FOXO1 A3 expression, Fig. ) for 30 min. To inhibit TIE2 phosphorylation, HUVECs were pretreated with 1 μg ml −1 BAY-826 (Tocris, 6579) for 10 min before rhANGPT1 stimulation. .. Sub-confluent HUVECs were transduced with custom-made adenoviruses (Vector Biolabs) to overexpress the FLAG-tagged human FOXO1 A3 that cannot be inhibited by PI3K–AKT signaling and is, thus, constitutively nuclear.

    Article Title: Angiopoietin-TIE2 feedforward circuit promotes PIK3CA-driven venous malformations.
    Article Snippet: .. To stimulate TIE2 phosphorylation, rhANGPT1 (R&D Systems, 923-AN-025/CF) was reconstituted in PBS and used at the concentrations of 50 ng ml−1 (PIK3CAH1047R expression, Fig. 5e) or 200 ng ml−1 (FOXO1A3 expression, Fig. 5f) for 30 min. To inhibit TIE2 phosphorylation, HUVECs were pretreated with 1 μg ml−1 BAY-826 (Tocris, 6579) for 10 min before rhANGPT1 stimulation. .. Sub-confluent HUVECs were transduced with custom-made adenoviruses (Vector Biolabs) to overexpress the FLAG-tagged human FOXO1A3 that cannot be inhibited by PI3K–AKT signaling and is, thus, constitutively nuclear.

    Expressing:

    Article Title: Angiopoietin–TIE2 feedforward circuit promotes PIK3CA-driven venous malformations
    Article Snippet: .. To stimulate TIE2 phosphorylation, rhANGPT1 (R&D Systems, 923-AN-025/CF) was reconstituted in PBS and used at the concentrations of 50 ng ml −1 (PIK3CAH1047R expression, Fig. ) or 200 ng ml −1 (FOXO1 A3 expression, Fig. ) for 30 min. To inhibit TIE2 phosphorylation, HUVECs were pretreated with 1 μg ml −1 BAY-826 (Tocris, 6579) for 10 min before rhANGPT1 stimulation. .. Sub-confluent HUVECs were transduced with custom-made adenoviruses (Vector Biolabs) to overexpress the FLAG-tagged human FOXO1 A3 that cannot be inhibited by PI3K–AKT signaling and is, thus, constitutively nuclear.

    Article Title: Angiopoietin-TIE2 feedforward circuit promotes PIK3CA-driven venous malformations.
    Article Snippet: .. To stimulate TIE2 phosphorylation, rhANGPT1 (R&D Systems, 923-AN-025/CF) was reconstituted in PBS and used at the concentrations of 50 ng ml−1 (PIK3CAH1047R expression, Fig. 5e) or 200 ng ml−1 (FOXO1A3 expression, Fig. 5f) for 30 min. To inhibit TIE2 phosphorylation, HUVECs were pretreated with 1 μg ml−1 BAY-826 (Tocris, 6579) for 10 min before rhANGPT1 stimulation. .. Sub-confluent HUVECs were transduced with custom-made adenoviruses (Vector Biolabs) to overexpress the FLAG-tagged human FOXO1A3 that cannot be inhibited by PI3K–AKT signaling and is, thus, constitutively nuclear.



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